Control:Article Title: Nucleic acid encoding anti-IL-17A antibodies
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their uses
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their use in treating autoimmune and inflammatory
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO; 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect®) 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their use in treating autoimmune and inflammatory disorders
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat #5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; 00011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat # MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Concentration Assay:Article Title: Nucleic acid encoding anti-IL-17A antibodies
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their uses
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their use in treating autoimmune and inflammatory
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat#5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO; 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect®) 1.1% solution; C0011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat# MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
Article Title: Anti-IL-17A antibodies and their use in treating autoimmune and inflammatory disorders
Article Snippet: .. The next morning, rmIL-17A (SEQ ID NO: 83, MW 31000) at 1 nM, rmIL-17A/F (R&D Systems® Cat #5390-IL; MW 30400) at 3 nM, rmIL-17F (SEQ ID NO: 84; MW 30000) at 30 nM, rratIL-17A (SEQ ID NO: 85; MW 31000) at 1 nM or control medium were added in a volume of 50 μl to triplicate wells in the presence of 50 μl of different concentrations of test antibodies (XAB4 or XAB1), control antibodies (Simulect® 1.1% solution; 00011, 831179) or control medium to reach the final volume of 200 μl/well and the final concentration of 1% fetal calf serum. .. Culture supernatants were collected after an incubation of 24 h and KC production was measured by ELISA. (i) ELISA for Detection of Mouse KC Production ELISA microtiter plates were coated with a rat anti-mouse KC MAb (R&D Systems® Cat # MAB453; 100 μl/well at 1 μg/ml) in PBS/0.02% NaN3 and incubated overnight at 4° C. The following day, microtiter plates were blocked with 300 μl of PBS/2% BSA/0.02% NaN3 for 3 h at room temperature.
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